A pegRNA designer for CRISPR prime editing.

Prime editing tools that consist of a reverse transcriptase linked with Cas9 nickase are capable of generating targeted insertions, deletions, and base conversions without producing DNA double strand breaks or requiring any donor DNA.

You can see the alignment results of input sequences.

PAM Type

CRISPR-Cas orthologues for prime editing

Target Genome

Organism Type



Send request for a new organism.

Please specify how to download the reference genome 2bit or FASTA files of the organism.

Target Sequence

Reference sequences (maximum 1000 chars):

Expected sequences after prime editing (maximum 1000 chars):

crRNA length
(length of target without PAM)