A pegRNA designer for CRISPR prime editing.

Prime editing tools that consist of a reverse transcriptase linked with Cas9 nickase are capable of generating targeted insertions, deletions, and base conversions without producing DNA double strand breaks or requiring any donor DNA.

"Citation info: ""Hwang G.H. "et al". PE-Designer and PE-Analyzer: web-based design and analysis tools for CRISPR prime editing "Nucleic Acid Resaerch", gkab319 (2021)."

You can see the alignment results of input sequences.

PAM Type

CRISPR-Cas orthologues for prime editing

Target Genome

Organism Type



Send request for a new organism.

Please specify how to download the reference genome 2bit or FASTA files of the organism.

Target Sequence

Reference sequences (maximum 1000 chars):

Expected sequences after prime editing (maximum 1000 chars):

crRNA length
(length of target without PAM)