CRISPR RGEN Tools

You can see the alignment results of input sequences.

PAM Type

CRISPR-Cas orthologues for prime editing

SpCas9 from Streptococcus pyogenes: 5'-NGG-3'

SpCas9 from Streptococcus pyogenes: 5'-NGG-3' for target, 5'-NRG-3' for off-target (R = A or G)

VRER SpCas9 from Streptococcus pyogenes: 5'-NGCG-3'

VQR SpCas9 from Streptococcus pyogenes: 5'-NGA-3'

xCas9 3.7 (TLIKDIV SpCas9) from Streptococcus pyogenes: 5'-NGT-3'

Target Genome

Organism Type

Genomes

or,

Send request for a new organism.

Please specify how to download the reference genome 2bit or FASTA files of the organism.

Target Sequence

Reference sequence (maximum 1000 chars):

Induced sequence:

crRNA length
(length of target without PAM)

Submit

PE-Designer