CRISPR RGEN Tools

You can see the alignment results of input sequences.

PAM Type

CRISPR-Cas orthologues for prime editing

SpCas9 from Streptococcus pyogenes: 5'-NGG-3'

SpCas9 from Streptococcus pyogenes: 5'-NGG-3' for target, 5'-NRG-3' for off-target (R = A or G)

VRER SpCas9 from Streptococcus pyogenes: 5'-NGCG-3'

VQR SpCas9 from Streptococcus pyogenes: 5'-NGA-3'

SpRY Cas9 from Streptococcus pyogenes: 5'-NRY-3'

NG-Cas9 or xCas9 3.7 (TLIKDIV SpCas9) from Streptococcus pyogenes: 5'-NGN-3'

iSpyMac Cas9 from Streptococcus pyogenes and Streptococcus macacae: 5'-NAA-3'

SpRY Cas9 from Streptococcus pyogenes: 5'-NRN-3'

SpRY Cas9 from Streptococcus pyogenes: 5'-NYN-3'

Target Genome

Organism Type

Genomes

or,

Send request for a new organism.

Please specify how to download the reference genome 2bit or FASTA files of the organism.

Target Sequence

Reference sequences (maximum 1000 chars):

Expected sequences after prime editing (maximum 1000 chars):

crRNA length
(length of target without PAM)

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PE-Designer