Microhomology-based choice of Cas9 nuclease target sites.
- Pattern score: score of each pattern according to the microhomology size and the deletion length
- Microhomology score: the sum of all the pattern scores
- Out-of-frame score: the ratio of out-of-frame pattern scores over the sum of all the pattern scores
To avoid unwanted in-frame deletions in a protein-coding sequence as much as possible,
one should choose target sites with high out-of-frame scores. A score above 66 is recommended.