Optimal seletion of guide RNAs on the genome scale.
Cpf1-Database is a genome-wide gRNA library design tool for Cpf1. It contains all available targets of Acidaminococcus sp. Cpf1 (AsCpf1) or Lachnospiraceae bacterium Cpf1 (LbCpf1) that recognizes 5’-TTTN-3’ PAM sequences in all coding sequence (CDS) regions. Users can select optimal target sequences from thousands of genes at once simply by changing the filtering conditions (see tutorial here). In addition, please see a web application programming interface (web API) for information about advanced use. Current version of genome assemblies are noted here.
Citation info: Park J. and Bae S. Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1. Bioinformatics 34, 1077-1079 (2018)
Import genes from file...
You can upload a text file that contains gene symbols or Ensembl IDs seperated by line breaks or spaces.