Cas-OFFinder

A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases.







PAM Type

CRISPR/Cas-derived RNA-guided Endonucleases (RGENs)

Target Genome

Organism Type


Genomes


or,

Send request for a new organism.


Please specify how to download the reference genome 2bit or FASTA files of the organism.

Query Sequences

Please write crRNA sequences without PAM sequences (e.g. without NGG for SpCas9).
The length of each query sequence should be between 15 and 25 nt, and all be the same length!



Please note that large number of bulge size will significantly increase the calculation time!

Mixed bases are allowed.


(eq or less than)

(eq or less than)

(eq or less than)