Optimal seletion of guide RNAs at genome-scale.
Cas-Database is a genome-wide gRNA library design tool for Cas9 nucleases from Streptococcus pyogenes (SpCas9). It contains all available targets of SpCas9 nucleases that recognizes 5’-NGG-3’ PAM sequences in all coding sequence (CDS) regions. Users can select optimal target sequences from thousands of genes at once simply by changing the filtering conditions (see tutorial here). In addition, Cas-Database also provides a web application programming interface (web API) for advanced use.
Import genes from file...
You can upload a text file that contains gene symbols or Ensembl IDs seperated by line breaks or spaces.