Optimal seletion of guide RNAs on the genome-scale.
Cas-Database is a genome-wide gRNA library design tool for Cas9 nucleases from Streptococcus pyogenes (SpCas9). It contains all available targets of SpCas9 nucleases that recognizes 5’-NGG-3’ PAM sequences in all coding sequence (CDS) regions. Users can select optimal target sequences from thousands of genes at once simply by changing the filtering conditions (see tutorial here). In addition, Cas-Database also provides a web application programming interface (web API) for information about advanced use.
(Updated) Please click here to visit Cas-Database for Cpf1.
Citation info: Park J., Kim J.-S., and Bae S. Cas-Database: Web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9. Bioinformatics 32, 2017-2023 (2016).
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